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Popular pages
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TA cloning: Ultimate guide - Sharebiology
TA cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure in detail.
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DRBC agar: Principle and preparation - Sharebiology
Dichloran-rose bengal chloramphenicol agar or DRBC agar or DRBC media is used to enumerate microbes that spread, mostly fungi. In this article, we have discussed history, principle, and the media's pr...
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Primer Designing - Demonstration step by step - Sharebiology
Primer designing is the first and one of the critical steps for successful cloning. In this article, we have demonstrated the primer designing for various cloning methods.
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Ponceau S staining (preparation and protocol) - Sharebiology
Ponceau S staining solution preparation, staining and destaining procedures were discussed in detail.
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Laemmli buffer: Preparation (1x,2x & 4x) and principle - Sharebiology
The Laemmli sample buffer or Laemmli buffer is used for loading and better resolving of SDS-PAGE gels. In this article, you will learn the preparation and principle of the buffer in step-by-step.
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In-Fusion Cloning - Sharebiology
In-Fusion cloning is one of the most used methods for seamless cloning. In this article, you will learn history, applications, advantages, limitations, and the cloning procedure in detail.